human embryonic lung fibroblasts hfl1 Search Results


media human embryonic lung fibroblasts  (ATCC)
96
ATCC media human embryonic lung fibroblasts
Media Human Embryonic Lung Fibroblasts, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human embryonic fibroblast cell line hfl1  (China Center for Type Culture Collection)
90
China Center for Type Culture Collection human embryonic fibroblast cell line hfl1
Macrophage‐derived exosomes modulate myofibroblast differentiation in vitro. (A) Exosomes derived from RAW264.7 cells were labelled with PKH26 dye and then incubated with <t>HFL1</t> cells for 24 h. Scale bar: 20 μm. (B, D‐E) Western blot (B) and RT‐qPCR (D‐E) analyses of collagen I, α‐SMA and GAPDH in HFL1 cells treated with PBS, NC‐Exos, SiO 2 ‐Exos or SiO 2 + GW4869‐Exos were performed. These exosomes were derived from THP‐1 cells. (C, F‐G) Western blot (C) and RT‐qPCR (F‐G) analyses of collagen I, α‐SMA and GAPDH in NIH‐3T3 cells incubated with PBS, NC‐Exos, SiO 2 ‐Exos or SiO 2 + GW4869‐Exos were performed. These exosomes were derived from RAW264.7 cells. Student's t test; * P < .05, ** P < .01
Human Embryonic Fibroblast Cell Line Hfl1, supplied by China Center for Type Culture Collection, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human embryonic fibroblast cell line hfl1 - by Bioz Stars, 2026-10
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human embryonic lung fibroblasts hfl 1  (ATCC)
94
ATCC human embryonic lung fibroblasts hfl 1
Macrophage‐derived exosomes modulate myofibroblast differentiation in vitro. (A) Exosomes derived from RAW264.7 cells were labelled with PKH26 dye and then incubated with <t>HFL1</t> cells for 24 h. Scale bar: 20 μm. (B, D‐E) Western blot (B) and RT‐qPCR (D‐E) analyses of collagen I, α‐SMA and GAPDH in HFL1 cells treated with PBS, NC‐Exos, SiO 2 ‐Exos or SiO 2 + GW4869‐Exos were performed. These exosomes were derived from THP‐1 cells. (C, F‐G) Western blot (C) and RT‐qPCR (F‐G) analyses of collagen I, α‐SMA and GAPDH in NIH‐3T3 cells incubated with PBS, NC‐Exos, SiO 2 ‐Exos or SiO 2 + GW4869‐Exos were performed. These exosomes were derived from RAW264.7 cells. Student's t test; * P < .05, ** P < .01
Human Embryonic Lung Fibroblasts Hfl 1, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human fetal lung fibroblast cells mrc-5  (BioResource International Inc)
90
BioResource International Inc human fetal lung fibroblast cells mrc-5
Macrophage‐derived exosomes modulate myofibroblast differentiation in vitro. (A) Exosomes derived from RAW264.7 cells were labelled with PKH26 dye and then incubated with <t>HFL1</t> cells for 24 h. Scale bar: 20 μm. (B, D‐E) Western blot (B) and RT‐qPCR (D‐E) analyses of collagen I, α‐SMA and GAPDH in HFL1 cells treated with PBS, NC‐Exos, SiO 2 ‐Exos or SiO 2 + GW4869‐Exos were performed. These exosomes were derived from THP‐1 cells. (C, F‐G) Western blot (C) and RT‐qPCR (F‐G) analyses of collagen I, α‐SMA and GAPDH in NIH‐3T3 cells incubated with PBS, NC‐Exos, SiO 2 ‐Exos or SiO 2 + GW4869‐Exos were performed. These exosomes were derived from RAW264.7 cells. Student's t test; * P < .05, ** P < .01
Human Fetal Lung Fibroblast Cells Mrc 5, supplied by BioResource International Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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a549  (ATCC)
99
ATCC a549
Macrophage‐derived exosomes modulate myofibroblast differentiation in vitro. (A) Exosomes derived from RAW264.7 cells were labelled with PKH26 dye and then incubated with <t>HFL1</t> cells for 24 h. Scale bar: 20 μm. (B, D‐E) Western blot (B) and RT‐qPCR (D‐E) analyses of collagen I, α‐SMA and GAPDH in HFL1 cells treated with PBS, NC‐Exos, SiO 2 ‐Exos or SiO 2 + GW4869‐Exos were performed. These exosomes were derived from THP‐1 cells. (C, F‐G) Western blot (C) and RT‐qPCR (F‐G) analyses of collagen I, α‐SMA and GAPDH in NIH‐3T3 cells incubated with PBS, NC‐Exos, SiO 2 ‐Exos or SiO 2 + GW4869‐Exos were performed. These exosomes were derived from RAW264.7 cells. Student's t test; * P < .05, ** P < .01
A549, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human hfl1 cell line  (iCell Bioscience Inc)
90
iCell Bioscience Inc human hfl1 cell line
Macrophage‐derived exosomes modulate myofibroblast differentiation in vitro. (A) Exosomes derived from RAW264.7 cells were labelled with PKH26 dye and then incubated with <t>HFL1</t> cells for 24 h. Scale bar: 20 μm. (B, D‐E) Western blot (B) and RT‐qPCR (D‐E) analyses of collagen I, α‐SMA and GAPDH in HFL1 cells treated with PBS, NC‐Exos, SiO 2 ‐Exos or SiO 2 + GW4869‐Exos were performed. These exosomes were derived from THP‐1 cells. (C, F‐G) Western blot (C) and RT‐qPCR (F‐G) analyses of collagen I, α‐SMA and GAPDH in NIH‐3T3 cells incubated with PBS, NC‐Exos, SiO 2 ‐Exos or SiO 2 + GW4869‐Exos were performed. These exosomes were derived from RAW264.7 cells. Student's t test; * P < .05, ** P < .01
Human Hfl1 Cell Line, supplied by iCell Bioscience Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human hfl1 cell line - by Bioz Stars, 2026-10
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human monocyte leukaemia cell line thp-1  (China Center for Type Culture Collection)
90
China Center for Type Culture Collection human monocyte leukaemia cell line thp-1
Macrophage‐derived exosomes modulate myofibroblast differentiation in vitro. (A) Exosomes derived from RAW264.7 cells were labelled with PKH26 dye and then incubated with <t>HFL1</t> cells for 24 h. Scale bar: 20 μm. (B, D‐E) Western blot (B) and RT‐qPCR (D‐E) analyses of collagen I, α‐SMA and GAPDH in HFL1 cells treated with PBS, NC‐Exos, SiO 2 ‐Exos or SiO 2 + GW4869‐Exos were performed. These exosomes were derived from THP‐1 cells. (C, F‐G) Western blot (C) and RT‐qPCR (F‐G) analyses of collagen I, α‐SMA and GAPDH in NIH‐3T3 cells incubated with PBS, NC‐Exos, SiO 2 ‐Exos or SiO 2 + GW4869‐Exos were performed. These exosomes were derived from RAW264.7 cells. Student's t test; * P < .05, ** P < .01
Human Monocyte Leukaemia Cell Line Thp 1, supplied by China Center for Type Culture Collection, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human monocyte leukaemia cell line thp-1 - by Bioz Stars, 2026-10
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h460 cell lines  (Sinovac BioTech)
90
Sinovac BioTech h460 cell lines
Macrophage‐derived exosomes modulate myofibroblast differentiation in vitro. (A) Exosomes derived from RAW264.7 cells were labelled with PKH26 dye and then incubated with <t>HFL1</t> cells for 24 h. Scale bar: 20 μm. (B, D‐E) Western blot (B) and RT‐qPCR (D‐E) analyses of collagen I, α‐SMA and GAPDH in HFL1 cells treated with PBS, NC‐Exos, SiO 2 ‐Exos or SiO 2 + GW4869‐Exos were performed. These exosomes were derived from THP‐1 cells. (C, F‐G) Western blot (C) and RT‐qPCR (F‐G) analyses of collagen I, α‐SMA and GAPDH in NIH‐3T3 cells incubated with PBS, NC‐Exos, SiO 2 ‐Exos or SiO 2 + GW4869‐Exos were performed. These exosomes were derived from RAW264.7 cells. Student's t test; * P < .05, ** P < .01
H460 Cell Lines, supplied by Sinovac BioTech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+embryonic+lung+fibroblasts+hfl1/h460+cell+lines/pmc05104218-248-5-11
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h460 cell lines - by Bioz Stars, 2026-10
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human lung carcinoma a549 cell line  (Sinovac BioTech)
90
Sinovac BioTech human lung carcinoma a549 cell line
Macrophage‐derived exosomes modulate myofibroblast differentiation in vitro. (A) Exosomes derived from RAW264.7 cells were labelled with PKH26 dye and then incubated with <t>HFL1</t> cells for 24 h. Scale bar: 20 μm. (B, D‐E) Western blot (B) and RT‐qPCR (D‐E) analyses of collagen I, α‐SMA and GAPDH in HFL1 cells treated with PBS, NC‐Exos, SiO 2 ‐Exos or SiO 2 + GW4869‐Exos were performed. These exosomes were derived from THP‐1 cells. (C, F‐G) Western blot (C) and RT‐qPCR (F‐G) analyses of collagen I, α‐SMA and GAPDH in NIH‐3T3 cells incubated with PBS, NC‐Exos, SiO 2 ‐Exos or SiO 2 + GW4869‐Exos were performed. These exosomes were derived from RAW264.7 cells. Student's t test; * P < .05, ** P < .01
Human Lung Carcinoma A549 Cell Line, supplied by Sinovac BioTech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+embryonic+lung+fibroblasts+hfl1/human+lung+carcinoma+a549+cell+line/pm27895756-49-5-11
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human lung carcinoma a549 cell line - by Bioz Stars, 2026-10
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human breast adenocarcinoma cells mcf-7  (BioResource International Inc)
90
BioResource International Inc human breast adenocarcinoma cells mcf-7
Macrophage‐derived exosomes modulate myofibroblast differentiation in vitro. (A) Exosomes derived from RAW264.7 cells were labelled with PKH26 dye and then incubated with <t>HFL1</t> cells for 24 h. Scale bar: 20 μm. (B, D‐E) Western blot (B) and RT‐qPCR (D‐E) analyses of collagen I, α‐SMA and GAPDH in HFL1 cells treated with PBS, NC‐Exos, SiO 2 ‐Exos or SiO 2 + GW4869‐Exos were performed. These exosomes were derived from THP‐1 cells. (C, F‐G) Western blot (C) and RT‐qPCR (F‐G) analyses of collagen I, α‐SMA and GAPDH in NIH‐3T3 cells incubated with PBS, NC‐Exos, SiO 2 ‐Exos or SiO 2 + GW4869‐Exos were performed. These exosomes were derived from RAW264.7 cells. Student's t test; * P < .05, ** P < .01
Human Breast Adenocarcinoma Cells Mcf 7, supplied by BioResource International Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human breast adenocarcinoma cells mcf-7 - by Bioz Stars, 2026-10
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nih 3t3 murine embryo fibroblast  (European Collection of Authenticated Cell Cultures)
90
European Collection of Authenticated Cell Cultures nih 3t3 murine embryo fibroblast
Effect of ITGA11 genetic modification on <t>fibroblast</t> features. (A) ITGA11 was knocked down using siRNA in HFL‐1 and proteins were extracted and subjected to western blot analysis to detect the expression levels of each target (see Section ). Vertical axis: (B) ITGA11, (C) COL11A1, (D) integrin α5, (E) integrin β1, (F) fibronectin, and (G) α‐SMA protein expression normalized to β‐actin, (H) p‐ERK1/2 protein expression normalized to t‐ERK1/2 and (I) p‐SMAD3 protein expression normalized to t‐SMAD3. (J) Migration toward fibronectin (20 µg·mL −1 ) was assessed after silencing ITGA11 . Vertical axis: number of migrated cells per 5HPF. (K) Migration toward collagen type I (1 µg·mL −1 ) was assessed after silencing ITGA11 . (B‐K; n ≥ 3, one‐way ANOVA) Vertical axis: number of migrated cells per 5HPF. (L) <t>NIH</t> <t>3T3</t> cells were transfected with ITGA11 ‐overexpression vector or GFP and subjected to western blot analysis to detect the ITGA11 and α‐SMA expression levels (see Section ). (M) Migration toward fibronectin (20 µg·mL −1 ) was assessed with ITGA11 ‐overexpressing NIH 3T3 cells. (M; n = 8, Mann–Whitney) Vertical axis: number of migrated cells per 5HPF. The values represent the mean ± SD. * P < 0.05, ** P < 0.01, **** P < 0.0001.
Nih 3t3 Murine Embryo Fibroblast, supplied by European Collection of Authenticated Cell Cultures, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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actin  (Valiant Co Ltd)
96
Valiant Co Ltd actin
Effect of ITGA11 genetic modification on <t>fibroblast</t> features. (A) ITGA11 was knocked down using siRNA in HFL‐1 and proteins were extracted and subjected to western blot analysis to detect the expression levels of each target (see Section ). Vertical axis: (B) ITGA11, (C) COL11A1, (D) integrin α5, (E) integrin β1, (F) fibronectin, and (G) α‐SMA protein expression normalized to β‐actin, (H) p‐ERK1/2 protein expression normalized to t‐ERK1/2 and (I) p‐SMAD3 protein expression normalized to t‐SMAD3. (J) Migration toward fibronectin (20 µg·mL −1 ) was assessed after silencing ITGA11 . Vertical axis: number of migrated cells per 5HPF. (K) Migration toward collagen type I (1 µg·mL −1 ) was assessed after silencing ITGA11 . (B‐K; n ≥ 3, one‐way ANOVA) Vertical axis: number of migrated cells per 5HPF. (L) <t>NIH</t> <t>3T3</t> cells were transfected with ITGA11 ‐overexpression vector or GFP and subjected to western blot analysis to detect the ITGA11 and α‐SMA expression levels (see Section ). (M) Migration toward fibronectin (20 µg·mL −1 ) was assessed with ITGA11 ‐overexpressing NIH 3T3 cells. (M; n = 8, Mann–Whitney) Vertical axis: number of migrated cells per 5HPF. The values represent the mean ± SD. * P < 0.05, ** P < 0.01, **** P < 0.0001.
Actin, supplied by Valiant Co Ltd, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+embryonic+lung+fibroblasts+hfl1/Actin/custom%4008771012%4036095970
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actin - by Bioz Stars, 2026-10
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Image Search Results


Macrophage‐derived exosomes modulate myofibroblast differentiation in vitro. (A) Exosomes derived from RAW264.7 cells were labelled with PKH26 dye and then incubated with HFL1 cells for 24 h. Scale bar: 20 μm. (B, D‐E) Western blot (B) and RT‐qPCR (D‐E) analyses of collagen I, α‐SMA and GAPDH in HFL1 cells treated with PBS, NC‐Exos, SiO 2 ‐Exos or SiO 2 + GW4869‐Exos were performed. These exosomes were derived from THP‐1 cells. (C, F‐G) Western blot (C) and RT‐qPCR (F‐G) analyses of collagen I, α‐SMA and GAPDH in NIH‐3T3 cells incubated with PBS, NC‐Exos, SiO 2 ‐Exos or SiO 2 + GW4869‐Exos were performed. These exosomes were derived from RAW264.7 cells. Student's t test; * P < .05, ** P < .01

Journal: Journal of Cellular and Molecular Medicine

Article Title: Macrophage‐derived exosomes mediate silica‐induced pulmonary fibrosis by activating fibroblast in an endoplasmic reticulum stress‐dependent manner

doi: 10.1111/jcmm.16524

Figure Lengend Snippet: Macrophage‐derived exosomes modulate myofibroblast differentiation in vitro. (A) Exosomes derived from RAW264.7 cells were labelled with PKH26 dye and then incubated with HFL1 cells for 24 h. Scale bar: 20 μm. (B, D‐E) Western blot (B) and RT‐qPCR (D‐E) analyses of collagen I, α‐SMA and GAPDH in HFL1 cells treated with PBS, NC‐Exos, SiO 2 ‐Exos or SiO 2 + GW4869‐Exos were performed. These exosomes were derived from THP‐1 cells. (C, F‐G) Western blot (C) and RT‐qPCR (F‐G) analyses of collagen I, α‐SMA and GAPDH in NIH‐3T3 cells incubated with PBS, NC‐Exos, SiO 2 ‐Exos or SiO 2 + GW4869‐Exos were performed. These exosomes were derived from RAW264.7 cells. Student's t test; * P < .05, ** P < .01

Article Snippet: The mouse macrophage cell line RAW264.7, mouse embryonic fibroblast cell line NIH‐3T3, human monocyte leukaemia cell line THP‐1 and human embryonic fibroblast cell line HFL1 were purchased from the China Center for Type Culture Collection (CCTCC).

Techniques: Derivative Assay, In Vitro, Incubation, Western Blot, Quantitative RT-PCR

SiO 2 ‐Exos induce lung fibroblast proliferation and migration (A‐B) CCK‐8 assay was used to evaluate the viability of HFL1 cells (A) and NIH‐3T3 cells (B) treated with various exosomes (SiO 2 ‐Exos or SiO 2 + GW4869‐Exos). Two‐way ANOVA; ** P < .01, *** P < .001, n.s: not significant. (C‐D) The migration of HFL1 cells (C) and NIH‐3T3 cells (D) was assessed by wound closure assay. Scale bar: 100 μm. Student's t test; * P < .05, ** P < .01, *** P < .001, n.s: not significant

Journal: Journal of Cellular and Molecular Medicine

Article Title: Macrophage‐derived exosomes mediate silica‐induced pulmonary fibrosis by activating fibroblast in an endoplasmic reticulum stress‐dependent manner

doi: 10.1111/jcmm.16524

Figure Lengend Snippet: SiO 2 ‐Exos induce lung fibroblast proliferation and migration (A‐B) CCK‐8 assay was used to evaluate the viability of HFL1 cells (A) and NIH‐3T3 cells (B) treated with various exosomes (SiO 2 ‐Exos or SiO 2 + GW4869‐Exos). Two‐way ANOVA; ** P < .01, *** P < .001, n.s: not significant. (C‐D) The migration of HFL1 cells (C) and NIH‐3T3 cells (D) was assessed by wound closure assay. Scale bar: 100 μm. Student's t test; * P < .05, ** P < .01, *** P < .001, n.s: not significant

Article Snippet: The mouse macrophage cell line RAW264.7, mouse embryonic fibroblast cell line NIH‐3T3, human monocyte leukaemia cell line THP‐1 and human embryonic fibroblast cell line HFL1 were purchased from the China Center for Type Culture Collection (CCTCC).

Techniques: Migration, CCK-8 Assay, Wound Closure Assay

SiO 2 ‐Exo‐induced myofibroblast differentiation is ER stress dependent (A‐C, E) Western blot analysis of BIP, XBP1s, P ‐eIF2α, α‐SMA, Collagen I, and GAPDH in HFL1 cells (A, C) and NIH‐3T3 cells (B, E) incubated with SiO 2 ‐Exos (± 4‐PBA) at different points in time (0, 8, 16 or 24 h). (D, F) Analysis of collagen I, α‐SMA, and GAPDH in HFL1 cells (D) and NIH‐3T3 cells (F) incubated with SiO 2 ‐Exos or SiO 2 ‐Exos +4‐PBA for 24 h

Journal: Journal of Cellular and Molecular Medicine

Article Title: Macrophage‐derived exosomes mediate silica‐induced pulmonary fibrosis by activating fibroblast in an endoplasmic reticulum stress‐dependent manner

doi: 10.1111/jcmm.16524

Figure Lengend Snippet: SiO 2 ‐Exo‐induced myofibroblast differentiation is ER stress dependent (A‐C, E) Western blot analysis of BIP, XBP1s, P ‐eIF2α, α‐SMA, Collagen I, and GAPDH in HFL1 cells (A, C) and NIH‐3T3 cells (B, E) incubated with SiO 2 ‐Exos (± 4‐PBA) at different points in time (0, 8, 16 or 24 h). (D, F) Analysis of collagen I, α‐SMA, and GAPDH in HFL1 cells (D) and NIH‐3T3 cells (F) incubated with SiO 2 ‐Exos or SiO 2 ‐Exos +4‐PBA for 24 h

Article Snippet: The mouse macrophage cell line RAW264.7, mouse embryonic fibroblast cell line NIH‐3T3, human monocyte leukaemia cell line THP‐1 and human embryonic fibroblast cell line HFL1 were purchased from the China Center for Type Culture Collection (CCTCC).

Techniques: Western Blot, Incubation

Inhibition of ER stress attenuates SiO 2 ‐Exo‐induced lung fibroblast proliferation and migration (A‐B) CCK‐8 assay was used to evaluate the viability of HFL1 cells (A) and NIH‐3T3 cells (B) incubated with SiO 2 ‐Exos ± 4‐PBA. Two‐way ANOVA; * P < .05, ** P < .01, *** P < .001, n.s: not significant. (C‐D) The migration of HFL1 cells (C) and NIH‐3T3 cells (D) was assessed by wound closure assay. Scale bar: 100 μm. Student's t test; * P < .05, ** P < .01, *** P < .001, n.s: not significant

Journal: Journal of Cellular and Molecular Medicine

Article Title: Macrophage‐derived exosomes mediate silica‐induced pulmonary fibrosis by activating fibroblast in an endoplasmic reticulum stress‐dependent manner

doi: 10.1111/jcmm.16524

Figure Lengend Snippet: Inhibition of ER stress attenuates SiO 2 ‐Exo‐induced lung fibroblast proliferation and migration (A‐B) CCK‐8 assay was used to evaluate the viability of HFL1 cells (A) and NIH‐3T3 cells (B) incubated with SiO 2 ‐Exos ± 4‐PBA. Two‐way ANOVA; * P < .05, ** P < .01, *** P < .001, n.s: not significant. (C‐D) The migration of HFL1 cells (C) and NIH‐3T3 cells (D) was assessed by wound closure assay. Scale bar: 100 μm. Student's t test; * P < .05, ** P < .01, *** P < .001, n.s: not significant

Article Snippet: The mouse macrophage cell line RAW264.7, mouse embryonic fibroblast cell line NIH‐3T3, human monocyte leukaemia cell line THP‐1 and human embryonic fibroblast cell line HFL1 were purchased from the China Center for Type Culture Collection (CCTCC).

Techniques: Inhibition, Migration, CCK-8 Assay, Incubation, Wound Closure Assay

Effect of ITGA11 genetic modification on fibroblast features. (A) ITGA11 was knocked down using siRNA in HFL‐1 and proteins were extracted and subjected to western blot analysis to detect the expression levels of each target (see Section ). Vertical axis: (B) ITGA11, (C) COL11A1, (D) integrin α5, (E) integrin β1, (F) fibronectin, and (G) α‐SMA protein expression normalized to β‐actin, (H) p‐ERK1/2 protein expression normalized to t‐ERK1/2 and (I) p‐SMAD3 protein expression normalized to t‐SMAD3. (J) Migration toward fibronectin (20 µg·mL −1 ) was assessed after silencing ITGA11 . Vertical axis: number of migrated cells per 5HPF. (K) Migration toward collagen type I (1 µg·mL −1 ) was assessed after silencing ITGA11 . (B‐K; n ≥ 3, one‐way ANOVA) Vertical axis: number of migrated cells per 5HPF. (L) NIH 3T3 cells were transfected with ITGA11 ‐overexpression vector or GFP and subjected to western blot analysis to detect the ITGA11 and α‐SMA expression levels (see Section ). (M) Migration toward fibronectin (20 µg·mL −1 ) was assessed with ITGA11 ‐overexpressing NIH 3T3 cells. (M; n = 8, Mann–Whitney) Vertical axis: number of migrated cells per 5HPF. The values represent the mean ± SD. * P < 0.05, ** P < 0.01, **** P < 0.0001.

Journal: Molecular Oncology

Article Title: Cancer‐associated fibroblast migration in non‐small cell lung cancers is modulated by increased integrin α11 expression

doi: 10.1002/1878-0261.12937

Figure Lengend Snippet: Effect of ITGA11 genetic modification on fibroblast features. (A) ITGA11 was knocked down using siRNA in HFL‐1 and proteins were extracted and subjected to western blot analysis to detect the expression levels of each target (see Section ). Vertical axis: (B) ITGA11, (C) COL11A1, (D) integrin α5, (E) integrin β1, (F) fibronectin, and (G) α‐SMA protein expression normalized to β‐actin, (H) p‐ERK1/2 protein expression normalized to t‐ERK1/2 and (I) p‐SMAD3 protein expression normalized to t‐SMAD3. (J) Migration toward fibronectin (20 µg·mL −1 ) was assessed after silencing ITGA11 . Vertical axis: number of migrated cells per 5HPF. (K) Migration toward collagen type I (1 µg·mL −1 ) was assessed after silencing ITGA11 . (B‐K; n ≥ 3, one‐way ANOVA) Vertical axis: number of migrated cells per 5HPF. (L) NIH 3T3 cells were transfected with ITGA11 ‐overexpression vector or GFP and subjected to western blot analysis to detect the ITGA11 and α‐SMA expression levels (see Section ). (M) Migration toward fibronectin (20 µg·mL −1 ) was assessed with ITGA11 ‐overexpressing NIH 3T3 cells. (M; n = 8, Mann–Whitney) Vertical axis: number of migrated cells per 5HPF. The values represent the mean ± SD. * P < 0.05, ** P < 0.01, **** P < 0.0001.

Article Snippet: HFL‐1 human fetal lung fibroblasts (catalog no. CCL‐153) and A549 human lung adenocarcinoma (catalog no. CCL‐185) cell lines were purchased from American Type Culture Collection (Manassas, VA, USA); NIH 3T3 murine embryo fibroblast (catalog no. EC93061524‐F0), and BEAS‐2B human normal bronchial epithelium (catalog no. EC95102433) were purchased from European Collection of Authenticated Cell Cultures (Salisbury, UK).

Techniques: Modification, Western Blot, Expressing, Migration, Transfection, Over Expression, Plasmid Preparation, MANN-WHITNEY